Saccharification of pineapple peelings through dilute acid hydrolysis

It has reported avoiding substrate inhibition, lower substrate concentrations are more appropriate. The diluted acid pretreatment methods have been developed in different types of reactors including percolation, plug flow, shrinking-bed, batch, flow-through reactor and countercurrent reactors [ 40 ].

Optimizing Clarification of Pineapple Peel

In our study, only nonmodified yeasts strains were used. Biotechnology Advances, 30, — Lignocellulose biomass as the most abundant biopolymer on earth is a potential renewable resource for the production of bioethanol.

Depletion of fossil fuels and anthropogenic climate change-A review.

Bioethanol Production from Pineapple (Ananas ...

Hydrolysis by Acid and Saccharification by Enzyme: During the fermentation, ethanol concentration yields of the fermentation broths inoculated with S. Research Article Cellulase production by Aspergillus niger under solid state fermentation using agro industrial wastes.

BioResources, 3 1The next stage of the investigation focused on whether pretreatment of the ground beet pulp improved the release of fermentable sugars. The presence of different polymeric substances in cell walls justify the need of a pre-treatment on this waste.

With the same adding of acetic acid, different crystallinity and pore structure are obtained through change the order of addition of acetic acid.

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The distillation apparatus with around-bottom flask containing the fermentation broth was attached at one end and a receiving flask at another end to collect the distillate. At the end of the 24 h, the crucibles were taken from the oven, allowed to cool in a desiccator at room temperature and reweighed.

The concentration of the reducing sugar present in the sample was estimated by adding 1 ml of DNS reagent to 1 ml of each of the sample then boiled for 5 mins and diluted with 10 ml of distilled water. Nutritional and environmental factors affecting cellulose production by two strains of cellulolytic bacilli.

Trends in Applied Sciences Research, 2: When the fermentation was complete, samples were collected to determine the ethanol, hexose, and pentose sugar concentrations. The microalgal biomass hydrolysates in this work were fermented by Aspergillus niger, Saccharomyces cerevisiae and co-culture of both.

Distilled water was used to blank the refractometer and then 3 mL of supernatant was drawn and dropped on a groove on the refractometer. Three different processes were used to obtain bioethanol: Development of improved cellulase mixtures in a single production organism.

The termination of the culture was to stop the organism from further hydrolysing the substrate. This was done in triplicate. Inexpensive carbon substrate like residual crude glycerol got after biodiesel production, agricultural waste converted sugars and cellulosic materials, trapped CO2 emitted from industries and molasses of sugar cane paved way to successful biomass production from microalgae.

Engineering plants and enzymes for biofuels production.

Journal of Energy

Ethanol fermentation from biomass resources: To carry-out the tests S. The main advantage of wet oxidation is efficient lignin removal and less inhibitor formations Table 1. The viable cells were counted and colony-forming units determined.

Evaluation of pretreatment with P. In aqueous solution, CO2 reacts with H2O to form carbonic acid and increases hydrolysis rate. From the study, it was revealed that citronella has lower cellulose content In the present work, the maximum TRS yield pretreated biomass was neutralized and water washed before enzymatic hydrolysis.

In dilute acid hydrolysis, it is the protons that break the heterocyclic ether bonds between the sugar monomers in the polymeric chains formed by hemicelluloses and cellulose (Mussatto &.

with increasing acid concentrations. Both the dilute and concentrated acid processes have several drawbacks. Dilute acid hydrolysis tends to yield some undesirable by-products in addition to the sugars [11]. They are furfural and 5-dihydroxymethyl furfural, which are known to inhibit fermentation [12].

Ethanol yield as a function of acetic acid concentration during simultaneous saccharification and fermentation (SSF) (average of acetic acid concentrations at 0 and 24 h).

The specific raw material demands and the straw to meal ratios for the process scenarios are listed in Table 4.


The best conditions for P. miczynskii β-glucosidase production were 3% pineapple peel as carbon source, under stationary conditions, for 9 days, at pH and temperature of 20°C, which yielded U/mL. The enzyme showed best activity at pH –, and 65°C.

All processes comprise the same main components: hydrolysis of the hemicellulose and the cellulose to monomer sugars, fermentation and product recovery and concentration by distillation. The main difference between the process alternatives is the hydrolysis steps, which can be performed by dilute acid, concentrated acid or en- zyme.

"Saccharification Of Pineapple Peelings Through Dilute Acid Hydrolysis" Essays and Research Papers Saccharification Of Pineapple Peelings Through Dilute Acid Hydrolysis Investigatory Project (Pineapple Peelings to Vinegar) Introduction A.

Background of the Study A Pineapple is a fruit wherein you have to peel off the peelings to eat it, like any other fruits.

Saccharification of pineapple peelings through dilute acid hydrolysis
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